Expression of prolactin and its receptor in the baboon uterus during the menstrual cycle and pregnancy.

نویسندگان

  • J Frasor
  • C A Gaspar
  • K M Donnelly
  • G Gibori
  • A T Fazleabas
چکیده

PRL is known to be a major secretory product of the human decidua. However, the physiological role of decidual PRL during the menstrual cycle and pregnancy has not been fully defined, primarily due to the lack of an appropriate nonhuman primate model for in vivo studies. Therefore, this initial study examined the expression of PRL and its receptor in the baboon uterus during the cycle and pregnancy. PRL and PRL receptor messenger ribonucleic acid expression were detected by semiquantitative RT-PCR, and protein was localized by immunocytochemistry. PRL was shown to be expressed in myometrial smooth muscle during the follicular phase by both RT-PCR and immunocytochemistry. Expression of PRL messenger ribonucleic acid and protein was first observed in the epithelial cells of the deep basal glands during the late luteal phase. With the onset of pregnancy, PRL expression increased steadily and was evident primarily in the decidual tissue. In contrast to PRL, its receptor was expressed at constant levels in both the myometrium and endometrium during the cycle. An increase in receptor expression was evident in both the decidua and placenta throughout pregnancy. In summary, these results demonstrate that the baboon uterus is a site of both PRL production and action during the cycle and pregnancy. These studies establish the baboon as a nonhuman primate model to investigate the potential roles of PRL in implantation and maintenance of pregnancy.

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

P-76: Important Role of Macrophage Colony-Stimulating Factor during Menstrual Cycle and Early Pregnancy Phase in Oovarian Stimulated IVF-Ppatients

Background: M-CSF is a cytokine mediating the growth, proliferation and differentiation of various cell types including macrophages, trophoblast cells, and osteoclasts. It plays an important role in immunity reproduction, follicle development and ovulation. In the present study, we describe the important role of the changes in serum M-CSF levels during the menstrual cycle, in the process of fol...

متن کامل

P-162: Pathophysiology of Endometriosis is Interacted by MIF, its Receptor and COX-2

Background: Endometriosis is a gynecological disease associated with severe pelvic pain and infertility. Immunological changes that occur in patients with endometriosis include reduced natural killer cell and T-lymphocyte cytotoxicity in the peritoneal fluid, and an elevated number of activated macrophages. MIF via its receptor, CD74, initiates a signaling cascade that leads to proliferation an...

متن کامل

The induction of baboon glycodelin expression by progesterone is not through Sp1.

Glycodelin is a major secretory product of the uterine glandular epithelial cells of the human and non-human primate during the late luteal phase of the menstrual cycle and early pregnancy. Since progesterone levels are elevated during these periods we sought to determine how progesterone modulates glycodelin gene expression. Co-transfection of various deletions of the baboon glycodelin promote...

متن کامل

P-217: Expression Analysis of The Histone Variant H2A.Z in Endometrium Tissue during The Menstrual Cycle

Background: The human endometrium undergoes cyclical changes including proliferation, differentiation, tissue breakdown and shedding (menstruation) throughout a woman’s reproductive life. The postovulatory rise in ovarian progestrone induces profound remodeling in chromatin structure of cells, and consequently differentiation of estradiol-primed endometrium. This change is crucial for embryo im...

متن کامل

The hormonal milieu in primary breast cancer: A correlation between steroid receptors and serum estradiol, progesterone and prolactin

Background: The female breast is subjected to a lifetime of hormonal controls, whose effect is evident at the time of menarche and during the menstrual cycle, pregnancy and lactation. Studies have reported multiple risk factors for breast cancer, some of which are a reflection of hormonally mediated events. The steroid receptors are also served as prognostic factors for evaluating status of mal...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:
  • The Journal of clinical endocrinology and metabolism

دوره 84 9  شماره 

صفحات  -

تاریخ انتشار 1999